Part:BBa_K299300
J23100[promoter].K299107[TxbaIG].J61100[RBS] No b-brick scars between
J23100[promoter].K299107[xbaIG].J61101[RBS] = Nucleotide sequence: T+xbaI+G 'binds' promoter and RBS instead of the biobrick scar.
Also be careful part J61100 is in registry as AAAGAGGGGACAA, and automattically when the B-brick suffix is added the next nucleotide generated by the registry page is 'T'. However the CORRECT J61101 sequence is:
AAAGAGGGGACAA**a** (and then comes the rest of the suffix: ctagt)
It means that the physical sequence is different to the record in registry for J61100, currently there is no way around it, because registry does not alow changes in suffix, and generates T anyway.
This part is just to enable automatic assembly of the Anderson's RBS collection in the original vector with parts cloned behind RBS. Anderson RBSes comes on PSB1A2 with additional promoter followed by T' XbaI G site and G. After this sequence the RBS is placed.
The original Anderson RBSes and vector are described here.
https://parts.igem.org/wiki/index.php?title=Part:BBa_J61100
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
- 1000COMPATIBLE WITH RFC[1000]
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