Measurement

Part:BBa_K299000:Design

Designed by: Anna Olchowik   Group: iGEM10_Warsaw   (2010-07-19)
Revision as of 00:14, 20 July 2010 by Olchowik (Talk | contribs) (→‎Design Notes)

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RBS measurement device J23100 J61100 GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

J23100 used here is part of the Anderon RBS biobrick J61100. (Anderson RBSes come in modified psb1a2 vectors)

...Biobrick Prefix..................J23100.................XbaI....RBS Part.....Biobrick Suffix... gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctagaGAAAGAGGGGACAAactagtagcggccgctgcag

Source

registry

References