Tag

Part:BBa_K416000

Designed by: Russell Durrett   Group: iGEM10_NYU   (2010-05-28)
Revision as of 17:01, 28 May 2010 by Registry (Talk | contribs)

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Aga2 CDS Responsible for Yeast Surface Display

The Aga2 protein is fused in frame with a protein that the user wants to display on the yeast cellular surface. Yeast cells constitutively expressing Aga1 (strain EBY100) secrete the Aga2 fusion protein, which associates with Aga1 through two disulfide bonds and quaternary structure to anchor Aga2 to the surface. The fused protein then is displayed on the surface of the yeast cell.

The STOP codon at the end of the CDS was removed to allow for N- and C-terminus fusions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
//proteindomain/localization
Parameters
None