SOC

Revision as of 18:49, 27 May 2010 by Meagan (Talk | contribs) (New page: == Summary == SOC Medium. Used in Bacterial Transformation. == Ingredients == *SOB *20 mM glucose ==Protocol== #Follow directions to make 1 liter of SOB media #After cooling...)

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Summary

SOC Medium. Used in Bacterial Transformation.

Ingredients

  • SOB
  • 20 mM glucose

Protocol

  1. Follow directions to make 1 liter of SOB media
  2. After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

The "C" in SOC supposedly stands for catabolite repression due to the added glucose.