Part:BBa_P1010

ccdB cell death gene

The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.

P1010 is used when putting BioBrick parts into BioBrick plasmids. The part to be inserted and the plasmid are cut with BioBrick enzymes and mixed. The mixture will include both the original uncut or religated plasmid and the desired structure. However, because of CcdB, all of the cells containing the original plasmid die and the surviving colonies are the desired result.

Also see P1016, a smaller part containing ccdB but not the inactive ccdA contained in P1010.

BioBrick Construction Plasmids Containing This Part

Most BioBrick plasmids are delivered with the P1010 insert see P1010 Physical DNA for the current list of plasmids that are available in this form. This particular Biobrick with its Plasmid backbone is considered a Construction Plasmid.

Usage and Biology

For more information on how to use this brick, visit its Featured Parts:Cell Death page

References

• "Positive-selection vectors using the F plasmid ccdB killer gene." Bernard P, Gabant P, Bahassi EM, Couturier M. 1994 Gene. Oct 11;148(1):71-4. [http://www.ncbi.nlm.nih.gov/pubmed/7926841 Pubmed]

• US Patent Number 5,910,438 "Cloning and/or sequencing vector" Bernard P, Gabant P, University Libre de Bruxelles, 1999. [http://www.google.com/patents?vid=USPAT5910438 Google Patents]
• US Patent Number 6,180,407 B1 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2001. [http://www.google.com/patents?vid=USPAT6180407 Google Patents]
• US Patent Number 7,176,029 B2 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2007. [http://www.google.com/patents?vid=USPAT7176029 Google Patents]

• "Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA." Bahassi, et al. 1999 J. Biol. Chem. 274: 10936-10944 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10196173&query_hl=2&itool=pubmed_ExternalLink Pubmed]
• Help:Plasmids
• Part:BBa_P1010