Part:BBa_K5127010:Design

pBrer-Tes4

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1012
  Illegal EcoRI site found at 1024
  Illegal XbaI site found at 775
  Illegal XbaI site found at 1087
  Illegal SpeI site found at 750
  Illegal PstI site found at 345
  Illegal PstI site found at 1345
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1012
  Illegal EcoRI site found at 1024
  Illegal NheI site found at 781
  Illegal NheI site found at 804
  Illegal NheI site found at 1058
  Illegal NheI site found at 1081
  Illegal SpeI site found at 750
  Illegal PstI site found at 345
  Illegal PstI site found at 1345
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1012
  Illegal EcoRI site found at 1024
  Illegal BglII site found at 1316
  Illegal BamHI site found at 1018
  Illegal BamHI site found at 1030
  Illegal BamHI site found at 1615
  Illegal XhoI site found at 1624
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1012
  Illegal EcoRI site found at 1024
  Illegal XbaI site found at 775
  Illegal XbaI site found at 1087
  Illegal SpeI site found at 750
  Illegal PstI site found at 345
  Illegal PstI site found at 1345
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1012
  Illegal EcoRI site found at 1024
  Illegal XbaI site found at 775
  Illegal XbaI site found at 1087
  Illegal SpeI site found at 750
  Illegal PstI site found at 345
  Illegal PstI site found at 1345
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E. coli.

Source

N/A

References

Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., Pritzel, A., Ronneberger, O., Willmore, L., Ballard, A. J., Bambrick, J., Bodenstein, S. W., Evans, D. A., Hung, C. C., O'Neill, M., Reiman, D., Tunyasuvunakool, K., Wu, Z., Žemgulytė, A., Arvaniti, E., ... Jumper, J. M. (2024). Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature, 630(493-500). https://doi.org/10.1038/s41586-024-07487-w

Beabout, K., Ehrenworth Breedon, A. M., Blum, S. M., Miklos, A. E., Lux, M. W., Chávez, J. L., & Goodson, M. S. (2022). Detection of Bile Acids in Complex Matrices Using a Transcription Factor-Based Biosensor. ACS Biomaterials Science & Engineering, 9(9), 5151–5162. https://doi.org/10.1021/acsbiomaterials.2c01006