Composite

Part:BBa_K5127026:Design

Designed by: Chujing Wu   Group: iGEM24_BNDS-China   (2024-10-02)
Revision as of 10:23, 2 October 2024 by Enolyn1214 (Talk | contribs) (→‎Source)

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Constitutive promoter with IAA degradation enzyme


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2402
    Illegal PstI site found at 2219
    Illegal PstI site found at 2797
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2402
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 724
    Illegal PstI site found at 2219
    Illegal PstI site found at 2797
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2402
    Illegal BglII site found at 2411
    Illegal BglII site found at 2463
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2402
    Illegal PstI site found at 2219
    Illegal PstI site found at 2797
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2402
    Illegal PstI site found at 2219
    Illegal PstI site found at 2797
    Illegal NgoMIV site found at 670
    Illegal NgoMIV site found at 1661
    Illegal AgeI site found at 434
    Illegal AgeI site found at 1463
    Illegal AgeI site found at 1904
    Illegal AgeI site found at 2327
    Illegal AgeI site found at 2761
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To optimize the IAA degradation efficiency, we constructed a second plasmid that replaces the inducible T7 promoter with the constitutive promoter J23119 (Figure 1). This modification ensures continuous expression of the IAA degradation pathway, potentially enhancing its overall effectiveness.


Source

E.coli

References

N/A