Part:BBa_K5127024:Design

iacR-pIacR-GFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 732
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.

Source

N/A

References

d’Oelsnitz, S., Stofel, S. K., Love, J. D., & Ellington, A. D. (2024). Snowprint: a predictive tool for genetic biosensor discovery. Communications Biology, 7(1). https://doi.org/10.1038/s42003-024-05849-8

Liang, C., Xiong, D., Zhang, Y., Mu, S., & Tang, S.-Y. (2014). Development of a novel uric-acid-responsive regulatory system in Escherichia coli. Applied Microbiology and Biotechnology, 99(5), 2267–2275. https://doi.org/10.1007/s00253-014-6290-6

Wei, W., Liu, Y., Hou, Y., Cao, S., Chen, Z., Zhang, Y., Cai, X., Yan, Q., Li, Z., Yuan, Y., Wang, G., Zheng, X., & Hao, H. (2023). Psychological stress-induced microbial metabolite indole-3-acetate disrupts intestinal cell lineage commitment. Cell Metabolism. https://doi.org/10.1016/j.cmet.2023.12.026