Device

Part:BBa_K5127013

Designed by: Chujing Wu   Group: iGEM24_BNDS-China   (2024-10-02)
Revision as of 07:30, 2 October 2024 by Registry (Talk | contribs)

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Butyrate sensor with NOT gate (pPcha-ci-pLam-GFP)

This composite part conbines the pPcha-cI system with pLam-cI NOT gate to reverse the logic in expressing the downstream genes, in which GFP downstream will be inhibit under the existence of butyrate for the characterizaton.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 970
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 239
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None