Coding

Part:BBa_K197014:Design

Designed by: Gabriela Guzman Lopez Aguado   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 05:19, 22 October 2009 by JCAnderson (Talk | contribs) (→‎Design Notes)

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{AIDA-I}


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 56
    Illegal PstI site found at 339
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 56
    Illegal PstI site found at 339
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 56
    Illegal PstI site found at 339
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 56
    Illegal PstI site found at 339
    Illegal AgeI site found at 771
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

PCR O09ig310/O09ig309 on EHEC (1184bp, gp = frag1)
PCR O09ig308/O09ig311 on EHEC (287bp, gp = frag2)
----
PCR O09ig310/O09ig311 on mixed frags    (1450bp, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig310         {?AIDA-1 AtD?}
----
O09ig310    Forward PCR of {?AIDA-1 AtD?} (B09ig310)
AAGTGGAATTCATGAGATCTGCTGGCAATGTGTTAGTGGTG
O09ig309    Reverse internal oligo for {?AIDA-1 AtD?} (B09ig310)
ATGGTGGCTCTTGAGCCAGGTTTT
O09ig308    Forward internal oligo for {?AIDA-1 AtD?} (B09ig310)
AAAACCTGGCTCAAGAGCCACCAT
O09ig311    Reverse PCR of {?AIDA-1 AtD?} (B09ig310)
GTTAGGGATCCGAATTGCCACTTAATGCCAAC

References