Composite

Part:BBa_K197034:Design

Designed by: Jennifer Brophy   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 05:12, 22 October 2009 by JCAnderson (Talk | contribs) (→‎Design Notes)

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{Pbad.rbs.prepro.StrepTag}{mgfp-5}{GFP-LVA}{cl02365 AtD}(dblterm)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2412
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1249
    Illegal PstI site found at 2412
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2412
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2412
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2337
    Illegal SapI site found at 1005


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

BglBrick assembly

References