Collections/VEGFR-like
VEGFR-like
This year, the USTC iGEM teamlianjiewenben has utilized the competitive binding of vascular endothelial growth factor (VEGF) to develop a targeted bacterial therapy for solid tumors. Our quest for the optimal VEGF-binding protein(or peptide) led us to an in-depth exploration of proteins structurally akin to the vascular endothelial growth factor receptor (VEGFR), which we have named VEGFR-like. In this year's project, we selected pBBR1MCS-2 as our plasmid backbone to construct a series of plasmids, which are used to validate the surface display system, VEGFR-like, and the masking peptides targeting VEGFR-like. And we have verified the expression of several of these plasmids in EcN.
| Name | Type | Description | Designer | Length |
| BBa_K5302000 | Coding | VEGFR1D2 | Jiacan Ma | 303 |
| BBa_K5302001 | Coding | 8IIU | Jiacan Ma | 378 |
| BBa_K5302002 | Coding | 8IJZ | Jiacan Ma | 378 |
| BBa_K5302003 | Coding | ZVEGF | Dekun Zhou | 177 |
| BBa_K5302004 | Coding | Z3C | Dekun Zhou | 177 |
| BBa_K5302005 | Coding | miniZ | Dekun Zhou | 102 |
| BBa_K5302006 | Coding | V114 | Dekun Zhou | 57 |
| BBa_K5302007 | Coding | V107 | Dekun Zhou | 57 |
| BBa_K5302008 | Coding | VEGFR-masking#18 | Dekun Zhou | 93 |
| BBa_K5302009 | Coding | VEGFR-masking#29 | Dekun Zhou | 84 |
| BBa_K5302010 | Coding | VGB | Dekun Zhou | 96 |
| BBa_K5302011 | Plasmid | pBBR-OmpA-mCherry | Jiacan Ma | 6483 |
| BBa_K5302012 | Plasmid | pBBR-INP-mcherry | Jiacan Ma | 6799 |
| BBa_K5302013 | Plasmid | pBBR-LppOmpA-mCherry | Bojun Yang | 5940 |
| BBa_K5302014 | Plasmid | pBBR-intimin-mCherry | Bojun Yang | 6957 |
| BBa_K5302015 | Plasmid | pBBR-AIDA-mCherry | Bojun Yang | 7269 |
| BBa_K5302016 | Plasmid | pBBR-OmpA-VEGFR1D2 | Xinyuan Shi | 6150 |
| BBa_K5302017 | Plasmid | pBBR-OmpA-8IIU | Xinyuan Shi | 6225 |
| BBa_K5302018 | Plasmid | pBBR-OmpA-8IJZ | Xinyuan Shi | 6225 |
| BBa_K5302019 | Plasmid | pBBR-OmpA-ZVEGF | Xinyuan Shi | 6024 |
| BBa_K5302020 | Plasmid | pBBR-OmpA-Z3C | Xinyuan Shi | 6024 |
| BBa_K5302021 | Plasmid | pBBR-OmpA-miniZ | Xinyuan Shi | 5949 |
| BBa_K5302022 | Plasmid | pBBR-OmpA-V114 | Xinyuan Shi | 5903 |
| BBa_K5302023 | Plasmid | pBBR-OmpA-V107 | Xinyuan Shi | 5903 |
| BBa_K5302024 | Plasmid | pBBR-INP-VEGFR1D2 | Bojun Yang | 6466 |
| BBa_K5302025 | Plasmid | pBBR-INP-8IIU | Bojun Yang | 6541 |
| BBa_K5302026 | Plasmid | pBBR-INP-8IJZ | Bojun Yang | 6541 |
| BBa_K5302027 | Plasmid | pBBR-INP-ZVEGF | Bojun Yang | 6340 |
| BBa_K5302028 | Plasmid | pBBR-INP-Z3C | Bojun Yang | 6340 |
| BBa_K5302029 | Plasmid | pBBR-INP-miniZ | Bojun Yang | 6265 |
| BBa_K5302030 | Plasmid | pBBR-INP-V114 | Bojun Yang | 6220 |
| BBa_K5302031 | Plasmid | pBBR-INP-V107 | Bojun Yang | 6220 |
| BBa_K5302032 | Plasmid | pBBR-OmpA-VEGFR1D2-l1masking#18 | Dekun Zhou | 6144 |
| BBa_K5302033 | Plasmid | pBBR-OmpA-VEGFR1D2-l2masking#18 | Dekun Zhou | 6144 |
| BBa_K5302034 | Plasmid | pBBR-OmpA-VEGFR1D2-l1masking#29 | Dekun Zhou | 6135 |
| BBa_K5302035 | Plasmid | pBBR-OmpA-VEGFR1D2-l2masking#29 | Dekun Zhou | 6135 |
| BBa_K5302036 | Plasmid | pBBR-OmpA-l1VGB | Dekun Zhou | 6147 |
| BBa_K5302037 | Plasmid | pBBR-OmpA-l2VGB | Dekun Zhou | 6147 |
| BBa_K5302038 | Plasmid | pBBR-INP-VEGFR1D2-l1masking#18 | Dekun Zhou | 6496 |
| BBa_K5302039 | Plasmid | pBBR-INP-VEGFR1D2-l2masking#18 | Dekun Zhou | 6496 |
| BBa_K5302040 | Plasmid | pBBR-INP-VEGFR1D2-l1masking#29 | Dekun Zhou | 6487 |
| BBa_K5302041 | Plasmid | pBBR-INP-VEGFR1D2-l2masking#29 | Dekun Zhou | 6487 |
| BBa_K5302042 | Plasmid | pBBR-INP-l1VGB | Dekun Zhou | 6499 |
| BBa_K5302043 | Plasmid | pBBR-INP-l2VGB | Dekun Zhou | 6499 |
Reference
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