Collections/VEGFR-like
Contents |
VEGFR-like
This year, the USTC iGEM team<a href="https://2024.igem.wiki/ustc/parts">lianjiewenben</a> has utilized the competitive binding of vascular endothelial growth factor (VEGF) to develop a targeted bacterial therapy for solid tumors. Our quest for the optimal VEGF-binding protein(or peptide) led us to an in-depth exploration of proteins structurally akin to the vascular endothelial growth factor receptor (VEGFR), which we have named VEGFR-like. In this year's project, we selected pBBR1MCS-2 as our plasmid backbone to construct a series of plasmids, which are used to validate the surface display system, VEGFR-like, and the masking peptides targeting VEGFR-like. And we have verified the expression of several of these plasmids in EcN.
| <a href=""></a>Name | <a href=""></a>Type | <a href=""></a>Description | <a href=""></a>Designer | <a href=""></a>Length |
| <a href="/Part:BBa_K5302000">BBa_K5302000</a> | <a href="">Coding</a> | <a href="">VEGFR1D2</a> | <a href="">Jiacan Ma</a> | <a href="">303</a> |
| <a href="https://parts.igem.org/Part:BBa_K5302001">BBa_K5302001</a> | <a href=""></a>Coding | <a href=""></a>8IIU | <a href=""></a>Jiacan Ma | <a href=""></a>378 |
| <a href="https://parts.igem.org/Part:BBa_K5302002">BBa_K5302002</a> | <a href=""></a>Coding | <a href=""></a>8IJZ | <a href=""></a>Jiacan Ma | <a href=""></a>378 |
| <a href="https://parts.igem.org/Part:BBa_K5302003">BBa_K5302003</a> | <a href=""></a>Coding | <a href=""></a>ZVEGF | <a href=""></a>Dekun Zhou | <a href=""></a>177 |
| <a href="https://parts.igem.org/Part:BBa_K5302004">BBa_K5302004</a> | <a href=""></a>Coding | <a href=""></a>Z3C | <a href=""></a>Dekun Zhou | <a href=""></a>177 |
| <a href="https://parts.igem.org/Part:BBa_K5302005">BBa_K5302005</a> | <a href=""></a>Coding | <a href=""></a>miniZ | <a href=""></a>Dekun Zhou | <a href=""></a>102 |
| <a href="https://parts.igem.org/Part:BBa_K5302006">BBa_K5302006</a> | <a href=""></a>Coding | <a href=""></a>V114 | <a href=""></a>Dekun Zhou | <a href=""></a>57 |
| <a href="https://parts.igem.org/Part:BBa_K5302007">BBa_K5302007</a> | <a href=""></a>Coding | <a href=""></a>V107 | <a href=""></a>Dekun Zhou | <a href=""></a>57 |
| <a href="https://parts.igem.org/Part:BBa_K5302008">BBa_K5302008</a> | <a href=""></a>Coding | <a href=""></a>VEGFR-masking#18 | <a href=""></a>Dekun Zhou | <a href=""></a>93 |
| <a href="https://parts.igem.org/Part:BBa_K5302009">BBa_K5302009</a> | <a href=""></a>Coding | <a href=""></a>VEGFR-masking#29 | <a href=""></a>Dekun Zhou | <a href=""></a>84 |
| <a href="https://parts.igem.org/Part:BBa_K5302010">BBa_K5302010</a> | <a href=""></a>Coding | <a href=""></a>VGB | <a href=""></a>Dekun Zhou | <a href=""></a>96 |
| <a href="https://parts.igem.org/Part:BBa_K5302011">BBa_K5302011</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-OmpA-mCherry | <a href=""></a>Jiacan Ma | <a href=""></a>6483 |
| <a href="https://parts.igem.org/Part:BBa_K5302012">BBa_K5302012</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-mcherry | <a href=""></a>Jiacan Ma | <a href=""></a>6799 |
| <a href="https://parts.igem.org/Part:BBa_K5302013">BBa_K5302013</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-LppOmpA-mCherry | <a href=""></a>Bojun Yang | <a href=""></a>5940 |
| <a href="https://parts.igem.org/Part:BBa_K5302014">BBa_K5302014</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-intimin-mCherry | <a href=""></a>Bojun Yang | <a href=""></a>6957 |
| <a href="https://parts.igem.org/Part:BBa_K5302015">BBa_K5302015</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-AIDA-mCherry | <a href=""></a>Bojun Yang | <a href=""></a>7269 |
| <a href="https://parts.igem.org/Part:BBa_K5302016">BBa_K5302016</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-OmpA-VEGFR1D2 | <a href=""></a>Xinyuan Shi | <a href=""></a>6150 |
| <a href="https://parts.igem.org/Part:BBa_K5302017">BBa_K5302017</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-OmpA-8IIU | <a href=""></a>Xinyuan Shi | <a href=""></a>6225 |
| <a href="https://parts.igem.org/Part:BBa_K5302018">BBa_K5302018</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-OmpA-8IJZ | <a href=""></a>Xinyuan Shi | <a href=""></a>6225 |
| <a href="https://parts.igem.org/Part:BBa_K5302019">BBa_K5302019</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-OmpA-ZVEGF | <a href=""></a>Xinyuan Shi | <a href=""></a>6024 |
| <a href="https://parts.igem.org/Part:BBa_K5302020">BBa_K5302020</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-OmpA-Z3C | <a href=""></a>Xinyuan Shi | <a href=""></a>6024 |
| <a href="https://parts.igem.org/Part:BBa_K5302021">BBa_K5302021</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-OmpA-miniZ | <a href=""></a>Xinyuan Shi | <a href=""></a>5949 |
| <a href="https://parts.igem.org/Part:BBa_K5302022">BBa_K5302022</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-OmpA-V114 | <a href=""></a>Xinyuan Shi | <a href=""></a>5903 |
| <a href="https://parts.igem.org/Part:BBa_K5302023">BBa_K5302023</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-OmpA-V107 | <a href=""></a>Xinyuan Shi | <a href=""></a>5903 |
| <a href="https://parts.igem.org/Part:BBa_K5302024">BBa_K5302024</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-VEGFR1D2 | <a href=""></a>Bojun Yang | <a href=""></a>6466 |
| <a href="https://parts.igem.org/Part:BBa_K5302025">BBa_K5302025</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-8IIU | <a href=""></a>Bojun Yang | <a href=""></a>6541 |
| <a href="https://parts.igem.org/Part:BBa_K5302026">BBa_K5302026</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-8IJZ | <a href=""></a>Bojun Yang | <a href=""></a>6541 |
| <a href="https://parts.igem.org/Part:BBa_K5302027">BBa_K5302027</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-ZVEGF | <a href=""></a>Bojun Yang | <a href=""></a>6340 |
| <a href="https://parts.igem.org/Part:BBa_K5302028">BBa_K5302028</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-Z3C | <a href=""></a>Bojun Yang | <a href=""></a>6340 |
| <a href="https://parts.igem.org/Part:BBa_K5302029">BBa_K5302029</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-miniZ | <a href=""></a>Bojun Yang | <a href=""></a>6265 |
| <a href="https://parts.igem.org/Part:BBa_K5302030">BBa_K5302030</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-V114 | <a href=""></a>Bojun Yang | <a href=""></a>6220 |
| <a href="https://parts.igem.org/Part:BBa_K5302031">BBa_K5302031</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-V107 | <a href=""></a>Bojun Yang | <a href=""></a>6220 |
| <a href="https://parts.igem.org/Part:BBa_K5302032">BBa_K5302032</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-OmpA-VEGFR1D2-l1masking#18 | <a href=""></a>Dekun Zhou | <a href=""></a>6144 |
| <a href="https://parts.igem.org/Part:BBa_K5302033">BBa_K5302033</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-OmpA-VEGFR1D2-l2masking#18</td> | <a href=""></a>Dekun Zhou</td> | <a href=""></a>6144</td>
</tr> |
| <a href="https://parts.igem.org/Part:BBa_K5302034">BBa_K5302034</a></td> | <a href=""></a>Plasmid</td> | <a href=""></a>pBBR-<i>OmpA-VEGFR1D2-l1masking#29</td> | <a href=""></a>Dekun Zhou</td> | <a href=""></a>6135</td>
</tr> |
| <a href="https://parts.igem.org/Part:BBa_K5302035">BBa_K5302035</a></td> | <a href=""></a>Plasmid</td> | <a href=""></a>pBBR-<i>OmpA-VEGFR1D2-l2masking#29</td> | <a href=""></a>Dekun Zhou</td> | <a href=""></a>6135</td>
</tr> |
| <a href="https://parts.igem.org/Part:BBa_K5302036">BBa_K5302036</a></td> | <a href=""></a>Plasmid</td> | <a href=""></a>pBBR-<i>OmpA-l1VGB</td> | <a href=""></a>Dekun Zhou</td> | <a href=""></a>6147</td>
</tr> |
| <a href="https://parts.igem.org/Part:BBa_K5302037">BBa_K5302037</a></td> | <a href=""></a>Plasmid</td> | <a href=""></a>pBBR-<i>OmpA-l2VGB</td> | <a href=""></a>Dekun Zhou</td> | <a href=""></a>6147</td>
</tr> |
| <a href="https://parts.igem.org/Part:BBa_K5302038">BBa_K5302038</a></td> | <a href=""></a>Plasmid</td> | <a href=""></a>pBBR-<i>INP-VEGFR1D2-l1masking#18 | <a href=""></a>Dekun Zhou | <a href=""></a>6496 |
| <a href="https://parts.igem.org/Part:BBa_K5302039">BBa_K5302039</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-VEGFR1D2-l2masking#18 | <a href=""></a>Dekun Zhou | <a href=""></a>6496 |
| <a href="https://parts.igem.org/Part:BBa_K5302040">BBa_K5302040</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-VEGFR1D2-l1masking#29 | <a href=""></a>Dekun Zhou | <a href=""></a>6487 |
| <a href="https://parts.igem.org/Part:BBa_K5302041">BBa_K5302041</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-VEGFR1D2-l2masking#29 | <a href=""></a>Dekun Zhou | <a href=""></a>6487 |
| <a href="https://parts.igem.org/Part:BBa_K5302042">BBa_K5302042</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-l1VGB | <a href=""></a>Dekun Zhou | <a href=""></a>6499 |
| <a href="https://parts.igem.org/Part:BBa_K5302043">BBa_K5302043</a> | <a href=""></a>Plasmid | <a href=""></a>pBBR-INP-l2VGB | <a href=""></a>Dekun Zhou | <a href=""></a>6499 |
Reference
[1]Ivan Guryanov*, Viktor Korzhikov-Vlakh, Madhushree Bhattacharya, Barbara Biondi, Giulia Masiero, Fernando Formaggio, Tatiana Tennikova, Arto Urtt. Conformationally Constrained Peptides with High Affinity to the Vascular Endothelial Growth Factor.Journal of Medicinal Chemistry. Vol 64/Issue 15.July 16, 2021
[2]Pan B, Li B, Russell SJ, Tom JY, Cochran AG, Fairbrother WJ. Solution structure of a phage-derived peptide antagonist in complex with vascular endothelial growth factor. J Mol Biol. 2002 Feb 22;316(3):769-87. doi: 10.1006/jmbi.2001.5370
[3]Checco JW, Kreitler DF, Thomas NC, Belair DG, Rettko NJ, Murphy WL, Forest KT, Gellman SH. Targeting diverse protein-protein interaction interfaces with α/β-peptides derived from the Z-domain scaffold. Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):4552-7. doi: 10.1073/pnas.1420380112
[4]Rossella Di Stasi, Donatella Diana, Domenica Capasso, Rosanna Palumbo, Alessandra Romanelli, Carlo Pedone, Roberto Fattorusso, Luca D. D'Andrea. VEGFR1D2 in drug discovery: Expression and molecular characterization.19 November 2010
[5]Anna Fedorova, Kerry Zobel, Herman S. Gill, Annie Ogasawara, Judith E. Flores, Jeff N. Tinianow, Alexander N. Vanderbilt, Ping Wu, Y. Gloria Meng, Simon-P. Williams, Christian Wiesmann, Jeremy Murray, Jan Marik, Kurt Deshayes. The Development of Peptide-Based Tools for the Analysis of Angiogenesis. Chemistry & Biology. Volume 18, Issue 7. 2011. Pages 839-845. ISSN 1074-5521
[6]Balsera B, Bonache MÁ, Reille-Seroussi M, Gagey-Eilstein N, Vidal M, González-Muñiz R, Pérez de Vega MJ. Disrupting VEGF-VEGFR1 Interaction: De Novo Designed Linear Helical Peptides to Mimic the VEGF13-25 Fragment. Molecules. 2017 Oct 28;22(11):1846
[7]Lei Wang,Lingyu Zhou,Marie Reille-Seroussi,Nathalie Gagey-Eilstein,Sylvain Broussy, Tianyu Zhang,Lili Ji,Michel Vidal*,Wang-Qing Liu.Identification of Peptidic Antagonists of Vascular Endothelial Growth Factor Receptor 1 by Scanning the Binding Epitopes of Its Ligands.July7.2017
[8]Sonia Nicchi1,2, Maria Giuliani1 , Fabiola Giusti1 , Laura Pancotto1 , Domenico Maione1 , Isabel Delany1 ,
Cesira L. Galeotti1 and Cecilia Brettoni1*.Decorating the surface of Escherichia coli with bacterial lipoproteins: a comparative analysis of diferent display systems.2021