Coding

Part:BBa_K5127002:Design

Designed by: Yinuo Li   Group: iGEM24_BNDS-China   (2024-10-01)
Revision as of 17:34, 1 October 2024 by Registry (Talk | contribs)

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BreR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 400
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 400
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 400
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 400
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.


Source

Genome of Vibrio cholerae

References