Composite

Part:BBa_K227012:Design

Designed by: WashU iGEM 2009   Group: iGEM09_Wash_U   (2009-10-17)
Revision as of 03:43, 22 October 2009 by Jathman (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

RBS34+OmpR(sphaeroides)+Term+ompC+pucBA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 587
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 629
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ompR(sph) is codon-optimized for use in R. sphaeroides. A search of R. sphaeroides revealed no EnvZ kinase homologues.[http://2009.igem.org/Team:Wash_U] When coupled with an introduced system containing an EnvZ kinase, this part would act as an appropriate reporter.

Source

composite part, synthesized in two fragments (RBS-Term, ompC+pucBA) from Geneart. Sequences from registry parts.

References