Composite

Part:BBa_K5323892:Design

Designed by: Yidan Hu   Group: iGEM24_CUG-China   (2024-10-01)
Revision as of 13:54, 1 October 2024 by Registry (Talk | contribs)

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Ptac-RBS-golS-Pgol-RBS-cI-TT-PR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1635
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3721
    Illegal AgeI site found at 1969
    Illegal AgeI site found at 2318
    Illegal AgeI site found at 3256
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to enable the normal expression of this gene in Acidithiobacillus ferrooxidans, we performed codon optimization.


Source

A composite part assembled by RBS(BBa_J34801), golS (BBa_K4468005), Pgol(BBa_K4468001), cI (BBa_R1051), Ptac(BBa_K180000), TT(BBa_B0015) and PR(BBa_K2229450).


References