Regulatory

Part:BBa_K5047036:Design

Designed by: Clément Alain Serge Corneil Vanmerris   Group: iGEM24_UNILausanne   (2024-09-30)
Revision as of 05:55, 1 October 2024 by Mwatson (Talk | contribs) (→‎Source)

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Sequence encodes for constitutive promoter pTEF1 from S. cerevisiae.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 160


Design Notes

None.


Source

The pTEF1 promoter originates from the pSP329 plasmid provided by Prof. Serge Pelet (DMF, University of Lausanne).

All common methods such as PCR, agarose gel electrophoresis, miniprep, and bacterial transformation are described in the Experiments section of the UniLausanne 2024 wiki page. https://2024.igem.wiki/unilausanne/experiments.

References