Device

Part:BBa_K173010:Experience

Designed by: Lorenzo Pasotti, Susanna Schiavi, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)
Revision as of 01:36, 22 October 2009 by Susanna (Talk | contribs)

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Please enter how you used this part and how it worked out.

Applications of BBa_K173010

This part was not working in a preliminary induction test.



Materials

  • Long term glycerol stocks were stored at -80°C with a final glycerol concentration of 20%
  • Antibiotics were: Ampicillin (Amp) 100 ug/ml, Kanamycin (Kan) 50 ug/ml and Chloramphenicol (Cm) 12.5 ug/ml. All of them were stored at -20°C in 1000x stocks. Amp and Kan were dissolved in water, while Cm was dissolved in ethanol 100%.
  • LB medium was prepare with: 1% NaCl, 1% bactotryptone, 0.5% yeast extract. The medium was not buffered with NaOH.
  • M9 supplemented medium was prepared according to: [http://openwetware.org/wiki/Knight:M9_supplemented_media Openwetware protocol].
  • 3OC6-HSL (Sigma) was dissolved in water and stored at -20°C in a 2mM stock.
  • aTc (Clontech) was dissolved in ethanol 50% and stored at -20°C in a 100 ug/ml stock. All the following dilutions were performed in water.
  • Ready made IPTG (Sigma) was stored at -20°C in a 200mM stock.


User Reviews

UNIQ17f66d5c32a5c0db-partinfo-00000000-QINU UNIQ17f66d5c32a5c0db-partinfo-00000001-QINU