Composite

Part:BBa_K5096056

Designed by: Matthew Murad   Group: iGEM24_Lambert-GA   (2024-09-30)
Revision as of 17:24, 30 September 2024 by Registry (Talk | contribs)

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dcas9 plasmid

The pCD017 plasmid encodes the dSpyCas9 protein, which is used in CRISPR interference (CRISPRi). dSpyCas9 is a deactivated form of Cas9 derived from Streptococcus pyogenes. dSpyCas9 lacks nuclease activity, therefore it cannot cut the DNA, but block the DNA Polymerase from initiating transcription. It does this by binding to single-guide RNA (sgRNA) to form a complex, effectively silencing the gene targeted by the sgRNA without introducing breaks in the DNA. This part was originally developed by Marshall et al. (2018).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4301
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4301
    Illegal NheI site found at 1220
    Illegal NheI site found at 4060
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4301
    Illegal BamHI site found at 6339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4301
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4301
    Illegal AgeI site found at 811
    Illegal AgeI site found at 1134
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 106


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Parameters
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