Composite

Part:BBa_K243028:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-19)
Revision as of 00:01, 22 October 2009 by Timo (Talk | contribs) (→‎Design Notes)

FOS-Split Linker-Fok_a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 766


Design Notes

The cloning steps were planned theoretically before we started the work in the wet lab. The combination of split linker, Fos and Fok_a allows an alternative method to bind our universal endonuclease protein to the DNA.

Source

Combined by serial cloning steps.

References