Composite

Part:BBa_K5097016

Designed by: Christopher Dorce   Group: iGEM24_Oneonta   (2024-09-25)
Revision as of 11:55, 30 September 2024 by Dorcc343 (Talk | contribs)

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sBFP with 6X His

Usage and Biology

The 2024 Oneonta iGEM team pHish and CHIPs used sBFP2 as a reporter protein to test the function of pH-sensitive regulatory elements that control gene expression. As a companion to these studies, we investigated what effect pH might have on the fluorescence of sBFP2. To do this, we generated a constitutively expressed sBFP2 (BBa_K156010) modified on the C-terminal to remove the native stop codon and added an AGVG linker and 6x His tag. A double-stop codon was added at the end.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 91


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