Part:BBa_K5324015

Destructin-1 Expression Device

This part is the Destructin-1 Expression Device that lets us see if Destructin-1 is active at the end of our sequence. The Destructin-1 (BBa_K5324015) is inserted into the circuit along with a His-tag (K112703) to see if Destructin-1 is still present at the end of the sequence. This device is enriched with a promoter, RBS, Short Linker, and terminator.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NotI site found at 102
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 879
  
• 1000
  COMPATIBLE WITH RFC[1000]