Part:BBa_K5241011

ADE2-gal1-egfp-CYC1

Short Description:

The ADE2 gene is combined with the yeast gal1 promoter, the enhanced green fluorescent protein gene egfp, and the CYC1 terminator.

genetic map：

Source:Brewing yeast

Description:

We used ADE2-gal1-egfp-CYC1 as the control group for ionizing radiation experiments, and Tyrosinase (BM) as the experimental group, to verify the radioprotective effect of melanin by comparison.

Result:

We conducted ionizing radiation experiments on ADE2-gal1-egfp-CYC1, generating a lethality curve as a control group.

Ionizing radiation time.	Control group. （ADE2-gal1-egfp-CYC1+）	Experimental group. （ADE2-gal1-egfp-CYC1+）	Viability of the microbial strain.
1h			48.91%
4h			28.54%
8h			5.92%
12h			0

In the next phase, we will expose tyrosinase (BM) to ionizing radiation and compare the differences between the two to assess the radiation-protective effect of melanin.

[1] Veronica J Heintz, Ling Wang, Douglas J LaCount, NanoLuc luciferase as a quantitative yeast two-hybrid reporter, FEMS Yeast Research, 10.1093/femsyr/foab069, 21, 8, (2021). View.

[2] David Cluet, Ikram Amri, Blandine Vergier, Jérémie Léault, Astrid Audibert, Clémence Grosjean, Dylan Calabrési, Martin Spichty, A Quantitative Tri-fluorescent Yeast Two-hybrid System: From Flow Cytometry to In cellula Affinities, Molecular & Cellular Proteomics, 10.1074/mcp.TIR119.001692, 19, 4, (701-715), (2020).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 4
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 201
  
• 1000
  COMPATIBLE WITH RFC[1000]