Part:BBa_K5241001

Pahdd

Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin synthesis.

Gene Circuit Diagram:

Source: Pseudomonas aeruginosa

Result：

Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm

Reference documentation

[1] ChiaraScanferla EnricoCaruso Viviana TeresaOrlandi FabrizioBolognese. Bacterial melanin production by heterologous expression of 4‑hydroxyphenylpyruvate dioxygenase from Pseudomonas aeruginosa[J]. International Journal of Biological Macromolecules, 2019, 133: 1072-1080.

[2] Zhang, Qihao, et al. "Pyomelanin Production via Heterologous Expression of 4-Hydroxyphenylpyruvate Dioxygenase (HPPD) and Construction of HPPD Inhibitor Screening Model." Journal of Bioscience and Bioengineering, vol. 136, no. 2, 2023, pp. 123-131. https://doi.org/10.1016/j.jbiosc.2023.01.003

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 56
  Illegal EcoRI site found at 227
  Illegal EcoRI site found at 701
  Illegal PstI site found at 932
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 56
  Illegal EcoRI site found at 227
  Illegal EcoRI site found at 701
  Illegal PstI site found at 932
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 56
  Illegal EcoRI site found at 227
  Illegal EcoRI site found at 701
  Illegal BglII site found at 936
  Illegal XhoI site found at 869
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 56
  Illegal EcoRI site found at 227
  Illegal EcoRI site found at 701
  Illegal PstI site found at 932
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 56
  Illegal EcoRI site found at 227
  Illegal EcoRI site found at 701
  Illegal PstI site found at 932
  
• 1000
  COMPATIBLE WITH RFC[1000]