Coding

Part:BBa_K194001

Designed by: Christian Kaas   Group: iGEM09_DTU_Denmark   (2009-07-13)
Revision as of 19:47, 21 October 2009 by Kaas (Talk | contribs)

GFP (a yeast- and FACS-optimized GFP variant),

This constitutively expressed GFP, originally from Aequorea victoria was codon optimized and developed as a reporter for gene expression in Saccharomyces cerevisiae according to Cormak et al (1997). In addition, two amino acid changes were included enabling a far more bright florescence compared to the wild type in E.coli. Maximum absorbance: 490 nm

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
Parameters
None