Composite

Part:BBa_K5366023

Designed by: Lin Li   Group: iGEM24_NJTECH-CHINA-A   (2024-09-27)
Revision as of 14:10, 27 September 2024 by Registry (Talk | contribs)

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T7 promoter-RBS-MBC-6xHis-T7 termonator

MBC structural gene expression plasmid

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 262
    Illegal PstI site found at 283
    Illegal PstI site found at 658
    Illegal PstI site found at 700
    Illegal PstI site found at 910
    Illegal PstI site found at 1468
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1543
    Illegal PstI site found at 262
    Illegal PstI site found at 283
    Illegal PstI site found at 658
    Illegal PstI site found at 700
    Illegal PstI site found at 910
    Illegal PstI site found at 1468
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 262
    Illegal PstI site found at 283
    Illegal PstI site found at 658
    Illegal PstI site found at 700
    Illegal PstI site found at 910
    Illegal PstI site found at 1468
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 262
    Illegal PstI site found at 283
    Illegal PstI site found at 658
    Illegal PstI site found at 700
    Illegal PstI site found at 910
    Illegal PstI site found at 1468
    Illegal NgoMIV site found at 1117
    Illegal AgeI site found at 406
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None