Coding

Part:BBa_K5241001

Designed by: yingmei tang   Group: iGEM24_ECIB-PKU   (2024-09-25)
Revision as of 06:26, 27 September 2024 by Supergrc (Talk | contribs)


Pahdd

Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin synthesis.

Gene Circuit Diagram:

Source: Pseudomonas aeruginosa

Result：Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal BglII site found at 936
    Illegal XhoI site found at 869
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None