Part:BBa_K5477030
XRE-pMEL1-NanoLuc reporter module
The XRE-pMEL1-NanoLuc reporter module is a critical component of the AhR (Aryl hydrocarbon receptor) biosensor system, designed to detect and report the presence of environmental toxins, such as polycyclic aromatic hydrocarbons (PAHs), dioxins and polychlorinated biphenyls (PCBs) (1) (2). In this module, the XRE (Xenobiotic Response Element) is the DNA sequence that AhR, once activated by ligand binding, specifically recognizes and binds to. The pMEL1 promoter, which is activated upon AhR binding to the XRE sequence, drives the expression of the NanoLuc reporter gene. NanoLuc, a highly sensitive and small luciferase enzyme, produces a bioluminescent signal under presence of contaminants.
The pUUS plasmid is a multi-copy plasmid used in yeast, carrying a URA3 marker for selection in uracil auxotrophic strains. In our system, pUUS is utilized in the reporter module, enabling the expression of the reporter gene, NanoLuc BBa_K1680009.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 59
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 59
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 59
Illegal BamHI site found at 3 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 59
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 59
- 1000COMPATIBLE WITH RFC[1000]
References
1. Carambia, A., Schuran, F.A. The aryl hydrocarbon receptor in liver inflammation. Semin Immunopathol 43, 563–575 (2021). https://doi.org/10.1007/s00281-021-00867-8
2. Mandal A, Biswas N, Alam MN. Implications of xenobiotic-response element(s) and aryl hydrocarbon receptor in health and diseases. Hum Cell. 2023 Sep;36(5):1638-1655. doi: 10.1007/s13577-023-00931-5. Epub 2023 Jun 17. PMID: 37329424.
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