Part:BBa_K5477029

pRET2-LexA-mERα(LBD) - receptor module

The pRET2-LexA-mERα(LBD) composite part is an receptor module that integrates the pRET2 promoter with a mutant version of the Estrogen Receptor Alpha ligand-binding domain (LBD), fused to the LexA DNA-binding domain (DBD). This fusion protein allows for the regulation of gene expression in response to bisphenol A (BPA) or other estrogen-like molecules, but with altered specificity and dynamics due to the mutation in the ERα LBD. In this system, the LexA- mERα(LBD) targets specific LexA operator sequences (Lex6Op) in our reporter module. The mutant ERα LBD (mERα) in this construct is designed to have altered ligand-binding properties compared to the wild-type ERα, allowing it to respond differently to ligands like BPA, which is known to act as an endocrine disruptor.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1606
  Illegal PstI site found at 1790
  Illegal PstI site found at 1961
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 182
  Illegal PstI site found at 1790
  Illegal PstI site found at 1961
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1740
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1606
  Illegal PstI site found at 1790
  Illegal PstI site found at 1961
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1606
  Illegal PstI site found at 1790
  Illegal PstI site found at 1961
  Illegal AgeI site found at 37
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1640