Part:BBa_K5477026
pRET2-NCOA - receptor module
The pRET2-NCOA composite part consists of the NCOA (Nuclear Receptor Coactivator) gene under the control of the pRET2 promoter, creating a module that enhances transcriptional activation by nuclear receptors like the Aryl hydrocarbon receptor (AhR). NCOA, also known as SRC (Steroid Receptor Coactivator), is a coactivator protein that interacts with activated nuclear receptors to promote transcription of target genes. NCOA achieves this by recruiting histone acetyltransferases (HATs) and other chromatin-remodeling complexes, which open the chromatin structure and allow transcription machinery to access DNA more effectively. In the pRET2-NCOA module, the pRET2 promoter drives the expression of NCOA, ensuring that it is available to function in pathways where enhanced nuclear receptor activity is required.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1785
Illegal EcoRI site found at 2407
Illegal SpeI site found at 1234
Illegal SpeI site found at 1535
Illegal SpeI site found at 2602
Illegal PstI site found at 1294 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1785
Illegal EcoRI site found at 2407
Illegal NheI site found at 182
Illegal NheI site found at 3547
Illegal SpeI site found at 1234
Illegal SpeI site found at 1535
Illegal SpeI site found at 2602
Illegal PstI site found at 1294 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1785
Illegal EcoRI site found at 2407
Illegal BamHI site found at 3049 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1785
Illegal EcoRI site found at 2407
Illegal SpeI site found at 1234
Illegal SpeI site found at 1535
Illegal SpeI site found at 2602
Illegal PstI site found at 1294 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1785
Illegal EcoRI site found at 2407
Illegal SpeI site found at 1234
Illegal SpeI site found at 1535
Illegal SpeI site found at 2602
Illegal PstI site found at 1294
Illegal AgeI site found at 37 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 3497
Illegal SapI.rc site found at 4999
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