Composite

Part:BBa_K5477026

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)
Revision as of 19:45, 26 September 2024 by Registry (Talk | contribs)

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pRET2-NCOA - receptor module

The pRET2-NCOA composite part consists of the NCOA (Nuclear Receptor Coactivator) gene under the control of the pRET2 promoter, creating a module that enhances transcriptional activation by nuclear receptors like the Aryl hydrocarbon receptor (AhR). NCOA, also known as SRC (Steroid Receptor Coactivator), is a coactivator protein that interacts with activated nuclear receptors to promote transcription of target genes. NCOA achieves this by recruiting histone acetyltransferases (HATs) and other chromatin-remodeling complexes, which open the chromatin structure and allow transcription machinery to access DNA more effectively. In the pRET2-NCOA module, the pRET2 promoter drives the expression of NCOA, ensuring that it is available to function in pathways where enhanced nuclear receptor activity is required.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1785
    Illegal EcoRI site found at 2407
    Illegal SpeI site found at 1234
    Illegal SpeI site found at 1535
    Illegal SpeI site found at 2602
    Illegal PstI site found at 1294
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1785
    Illegal EcoRI site found at 2407
    Illegal NheI site found at 182
    Illegal NheI site found at 3547
    Illegal SpeI site found at 1234
    Illegal SpeI site found at 1535
    Illegal SpeI site found at 2602
    Illegal PstI site found at 1294
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1785
    Illegal EcoRI site found at 2407
    Illegal BamHI site found at 3049
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1785
    Illegal EcoRI site found at 2407
    Illegal SpeI site found at 1234
    Illegal SpeI site found at 1535
    Illegal SpeI site found at 2602
    Illegal PstI site found at 1294
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1785
    Illegal EcoRI site found at 2407
    Illegal SpeI site found at 1234
    Illegal SpeI site found at 1535
    Illegal SpeI site found at 2602
    Illegal PstI site found at 1294
    Illegal AgeI site found at 37
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3497
    Illegal SapI.rc site found at 4999


[edit]
Categories
Parameters
None