Coding

Part:BBa_K5477016

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)
Revision as of 16:59, 26 September 2024 by Kateesc1700 (Talk | contribs)

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UGT2B15 - UDP-glucuronosyltransferase 2B15

UGT2B15 (UDP-glucuronosyltransferase 2B15) is an enzyme belonging to the UDP-glucuronosyltransferase (UGT) family, which plays a role in the phase II metabolism of xenobiotics and endogenous compounds. UGT2B15 is primarily involved in the process of glucuronidation, a biochemical reaction where glucuronic acid is transferred to lipophilic molecules, making them more water-soluble and easier to excrete from the body. This detoxification process is essential for eliminating harmful substances such as drugs, environmental toxins, and metabolic byproducts.

In particular, UGT2B15 is responsible for the glucuronidation of a wide range of compounds, including bisphenol A (BPA), steroid hormones (like androgens), and pharmaceuticals. By conjugating these substances with glucuronic acid, UGT2B15 facilitates their removal via urine or bile, reducing their biological activity and toxicity. The enzyme is expressed predominantly in the liver but is also found in other tissues such as the kidney, prostate, and gastrointestinal tract, where it participates in localized detoxification processes (1).

By incorporating UGT2B15 into the detoxification system, the goal is to metabolize BPA through glucuronidation, rendering it less harmful and easier to excrete from the cell or organism.

In our system, UGT2B15 was integrated in the following composites together with UDPD:


Results from Drylab

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1343
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1343
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1343
    Illegal BamHI site found at 1163
    Illegal BamHI site found at 1457
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1343
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1343
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 130


References

1. Ramírez, Viviana & Gálvez Ontiveros, Yolanda & Porras, Patricia & Martinez-Gonzalez, Luis & Rivas, Ana & Alvarez-Cubero, María. (2021). METABOLIC pathways, alterations in MIRNAS expression and effects of genetic polymorphisms of bisphenol a analogues: A SYSTEMATIC review. Environmental Research. 197. 111062. 10.1016/j.envres.2021.111062.

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