DNA

Part:BBa_K5492732

Designed by: Vojtech Vasut, Sandor Ban, Norbert Nyeste, Sara Simonka   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-15)
Revision as of 21:00, 25 September 2024 by Noriii (Talk | contribs)


S1_aptamer_reverse_complement_strand

ssDNA sequence designed to be complementary for the 5' end of s1_aptamer_with_fluorophore, it contains a quencher molecule (3'Iowa Black® RQ) at its 3' end, which absorbs the light emitted by the 5' fluorophore molecule (5' Cy5™) of the aptamer until it binds histamine.



Usage and Biology

Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.

Sequence and Features

aptamer-fluorophore.jpg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None