DNA

Part:BBa_K5492711

Designed by: Vojtech Vasut, Sandor Ban, Norbert Nyeste, Sara Simonka   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-15)
Revision as of 20:27, 25 September 2024 by Noriii (Talk | contribs)


J1_aptamer

J1 ssDNA aptamer sequence designed to specifically bind to histamine. The overhang of the 5' end of the sequence is complementary to j1_y1_aptamer_connector. The connector sequence contains a 5' biotin tag (5'biosg) that lets the sequence bind to an avidin-coated magnetic bead via avidin-biotin connection.


Usage and Biology

Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.

Sequence and Features

aptamer-beads-illustr-small.jpg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None