Tag

Part:BBa_K5097013

Designed by: Christopher Dorce   Group: iGEM24_Oneonta   (2024-09-25)
Revision as of 18:10, 25 September 2024 by Registry (Talk | contribs)

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BBa_K5097013 6X His tag with AGVG linker

This basic part codes for an 6X Histidine tag with a N-terminal AGVG linker and C terminal double stop codon. The part is intended for use in protein purification using IMAC (Immobilized Metal ion Affinity Chromatography) purification. This part can be added to the C-terminal of a coding sequence (with the native stop codon removed), which will add the flexible AGVG linker and then a 6X Histidine tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None