Composite

Part:BBa_K5080000

Designed by: Riva Yan   Group: iGEM24_Squirrel-Guangzhou   (2024-09-25)
Revision as of 14:02, 25 September 2024 by Registry (Talk | contribs)

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J23119-BBa_B0034-LuxR-BBa_B1006

LuxR and pLux form a well-established transcriptional regulatory protein and its corresponding promoter. In bacteria, once LuxR binds to AHL, the resulting complex binds to the pLux promoter region, initiating the expression of downstream genes. The concentration of AHL is related to bacterial population density, and when the concentration exceeds a certain threshold, the LuxR-AHL complex activates the expression of downstream genes. This plasmid uses the strong promoter J23119 to drive high levels of LuxR protein expression in Escherichia coli, allowing it to work with BBa_K5080001 to detect the concentration of AHL molecules in the environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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