RNA

Part:BBa_K5047000:Design

Designed by: Clement Alain Serge Corneil Vanmerris   Group: iGEM24_UNILausanne   (2024-09-22)
Revision as of 18:33, 24 September 2024 by Mwatson (Talk | contribs) (→‎Source)

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shRNAv1 : DNA sequence encodes a shRNA that does not bind to any genes. It serves as a control.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was chosen to lack any significant homology to functional genes in the model organism, thereby avoiding off-target effects making it an appropriate negative control.

Source

This part was synthetically designed. The main sequence was taken from a piece of DNA from Crispr interference. This part comes from Schearli Lab.

References