Composite

Part:BBa_K5299200

Designed by: Maria - Asimina Kakadiari   Group: iGEM24_Thessaly   (2024-09-23)
Revision as of 14:10, 23 September 2024 by Mkakadiari (Talk | contribs)


P3.1 - BBa_B0030 - BBa_I746916 - BBa_B0015

It consists of a promoter[1], a RBS, a sfGFP and a terminator.

It is able to produce the sfGFP according to the promoter's abilities.

It was constructed in order to check the strength of the P3.1 promoter. In the future, it can be used by other teams that wish to utilise the P3.1 promoter, in order to estimate that P3.1 is a suitable candidate in their design for their chassis of choice.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 259


References

  • [1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491
  • [2]: Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature Biotechnology, 24 (1), 79–88. doi:10.1038/nbt1172
[edit]
Categories
Parameters
None