Coding

Part:BBa_K5492402

Designed by: Norbert Nyeste, Sandor Ban, Vojtech Vasut, Sara Simonka   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-15)
Revision as of 11:26, 16 September 2024 by Noriii (Talk | contribs)


Wild type HNMT - pETDUET-1

Wild type coding sequence of human Histamine-N-Methyltransferase appropiated for insertion intopT7CFE1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal BglII site found at 1015
    Illegal BamHI site found at 123
    Illegal XhoI site found at 457
    Illegal XhoI site found at 1064
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
    Illegal NgoMIV site found at 1034
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None