Part:BBa_K5036008
NES
Part Description
It is a Nuclear Export Signal which is a short sequence of amino acids found in certain proteins that direct those proteins out of the nucleus of a cell. . In our model it is engineered in our second chain of the receptor
Usage
It help in increasing control over transcription activity by preventing delivery of dCas9 into the nucleus without activation of our syn dCas9RTK receptor
this figure illustrates the structure of NES in the second chain of syn dCas9RTK receptor .
Literature Characterization
Many studies suggest that protein movement between the cell nucleus and cytoplasm (nucleocytoplasmic translocation) is controlled by signals called NLS and NES. While NLS is known to be essential for Mst3 protein entering the nucleus, it's unclear if there's also an NES signal directing full-length Mst3 back out to the cytoplasm. To investigate this possibility, HeLa cells were temporarily modified with a construct expressing EGFP-tagged Mst3 (EGFP-Mst3WT) and then treated with LMB, a drug that blocks a specific protein (Crm1) involved in exporting proteins from the nucleus.
Figure A shows how the concentration of wild-type TEV protein compares to the L56V variant. A plateau in the absorption reading indicates the point at which the protein precipitates out of solution (limited solubility). Introducing mutations L56V and S135G significantly increased TEV solubility (Figure B), allowing them to reach maximum concentrations of 6.2 mg/mL and 5.77 mg/mL, respectively. Since the remaining variants (K45F, Q58F, E106G) displayed similar solubility to the wild-type TEV, they were not investigated further.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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