Coding

Part:BBa_K5492200:Design

Designed by: Vojtech Vasut, Norbert Nyeste   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-10)
Revision as of 18:19, 10 September 2024 by Registry (Talk | contribs)

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Diamine oxidase - DAO


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 55
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1932
    Illegal BamHI site found at 2186
    Illegal XhoI site found at 802
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for the following codons: AGA -> CGT, AGG -> CGT, ATA -> ATC, CCC -> CCG, CGG -> GCT, CTA -> CTG, GGA -> GGC


Source

The source of the part is its amino acid sequence which was retrieved from Uniprot (https://www.uniprot.org/uniprotkb/Q498N2/entry#sequences), and the work of iGEM20_SEHS-China (Part: BBa_K3684002) which provided the dna sequence we improved on.

References