Regulatory

Part:BBa_K5133000

Designed by: Fang Ba   Group: iGEM24_GEC-China   (2024-07-27)
Revision as of 02:35, 27 July 2024 by Bafang (Talk | contribs)


T7 promoter (from plasmid pJL1)

Group: GEC-China (iGEM 2024, team number: #5133)

This basic part is derived from plasmid pJL1 (Addgene: #69496)[1], including a conserved DNA sequence of T7 promoter as 5'-taatacgactcactatagggaga-3'[2]. The plasmid pJL1 is commonly used for the sfGFP expression for cell-free protein synthesis (CFPS). Hence, this part is used for the construction of three composite parts: BBa_K5133004 (sfGFP generator for CFPS), BBa_K5133006 (Microcin H47 generator for CFPS), and BBa_K5133008 (Microcin M generator for CFPS), respectively.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 51
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 51
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 32


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