MEASUREMENT
We measured the effect of inverter of Q04510 by test the response of these devices (these devices were transformed to :
K176112( J23115 + K176107);
K176113( J23109 + K176107);
K176114( J23103 + K176107);
OUTPUT: GFP
data
| COLONY
|
T(h)
|
OD600
|
FLU(arbitrary)
|
ST.FLU(ustc_st1)
|
| 1 |
3 |
0.170333333 |
0.457333333 |
1.827871037
|
| 2 |
3 |
0.155333333 |
0.457666667 |
1.829203304
|
| 1 |
4 |
0.283 |
0.777 |
3.105515588
|
| 2 |
4 |
0.262666667 |
0.532 |
2.126298961
|
| 1 |
5 |
0.401333333 |
1.129333333 |
4.513722355
|
| 2 |
5 |
0.401333333 |
0.864333333 |
3.454569678
|
| 1 |
6 |
0.512 |
1.726 |
6.898481215
|
| 2 |
6 |
0.532333333 |
1.316666667 |
5.262456701
|
| COLONY
|
T(h)
|
OD600
|
FLU(arbitrary)
|
ST.FLU(ustc_st1)
|
| 1 |
3 |
0.21 |
6.080333333 |
24.30189182
|
| 2 |
3 |
0.219 |
5.81 |
23.22142286
|
| 1 |
4 |
0.336666667 |
8.669666667 |
34.65094591
|
| 2 |
4 |
0.366 |
9.630333333 |
38.4905409
|
| 1 |
5 |
0.459 |
12.84733333 |
51.34825473
|
| 2 |
5 |
0.498 |
12.51266667 |
50.01065814
|
| 1 |
6 |
0.560666667 |
18.07833333 |
72.25552891
|
| 2 |
6 |
0.609 |
16.88866667 |
67.50066613
|
| COLONY
|
T(h)
|
OD600
|
FLU(arbitrary)
|
ST.FLU(ustc_st1)
|
| 1 |
3 |
0.150333333 |
52.47366667 |
209.7268852
|
| 2 |
3 |
0.127 |
53.24233333 |
212.7990941
|
| 1 |
4 |
0.263 |
135.7333333 |
542.4993339
|
| 2 |
4 |
0.221 |
132.8766667 |
531.0818012
|
| 1 |
5 |
0.390333333 |
230.72 |
922.1422862
|
| 2 |
5 |
0.339333333 |
222.6233333 |
889.7815081
|
| 1 |
6 |
0.515 |
314.0566667 |
1255.222489
|
| 2 |
6 |
0.459666667 |
299.9233333 |
1198.734346
|
For the details of the definition of ST.FLU and the unit ustc_st1, please click here.
plot
Figure 1-the relationship chart between ST.FLU and OD600. From the chart, we can see that the strength of the inverter is so strong that the expression of GFP is totally inhibited when the constitutive promoter is Pcon0.15 |
|
|
