Part:BBa_K4960027

pvc13 NTD-3*GGSGG-CKGGRAKDC-3*GGSGG-pvc13 CTD

A flexible linker which can influence the structure of tail fiber connected to the CKGGRAKDC.

Profile

Name:pvc13_NTD-3*GGSGG-CKGGRAKDC-3*GGSGG-pvc13_CTD
Base Pairs: 1422bp
Origin: Synthetic
Properties: A flexible linker which can influence the structure of tail fiber connected to the CKGGRAKDC.

Usage and Biology

As an indispensable component of recombinant fusion proteins, linkers have shown increasing importance in the construction of bioactive fusion proteins.[1] (GGGGS)n is a commonly used flexible linker.[1] Here, we use (GGGGS)3 to influence the structure of tail fiber connected to the CKGGRAKDC.(Figure 1)

Figure 1. AlphaFold2-guided Design of Adipose Cell-targeting PVC Coat Expressing Plasmids. AlphaFold2 based prediction of engineered PVC tail fiber trimer structure. Structure of adipose-targeting CKGGRAKDC peptide-presenting PVC tail fiber with indicated linkers were shown.

Special Design

Extend the flexible linker on both sides of the CKGGRAKDC to make the CKGGRAKDC more visibly exposed, which in turn facilitates more precise targeting of adipocytes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 309
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Chen, X., Zaro, J. L., & Shen, W. C. (2013). Fusion protein linkers: property, design and functionality. Advanced drug delivery reviews, 65(10), 1357–1369.