Reporter

Part:BBa_K216009:Experience

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-10-16)
Revision as of 16:29, 20 October 2009 by Cfrench (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K216009

This part was designed to characterize the activity of the PyeaR promoter by Miller assay, as a backup to the GFP-based construct used in the promoter characterization kit.

User Reviews

Initial Tests: Edinburgh iGEM 2009: To test this part, we grew E. coli JM109 overnight at 37 C with shaking in LB with 80 mg/l ampicillin and varying concentrations of sodium nitrate and sodium nitrite. The following day, we performed Miller assays (LacZ activity assays using o-nitrophenyl galactoside, ONPG, as a chromogenic substrate). The results showed strong induction by both nitrate and nitrite, as expected for this promoter (see biology information for part BBa_K216005).

UNIQ26d2a1f541d8cc51-partinfo-00000000-QINU UNIQ26d2a1f541d8cc51-partinfo-00000001-QINU