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Part:BBa_K1598002:Experience

Designed by: Marta Napiorkowska   Group: iGEM15_UCL   (2015-07-22)
Revision as of 01:02, 12 October 2023 by Zcornzhou (Talk | contribs) (Squirrel-CHN-II 2023)


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Applications of BBa_K1598002

Squirrel-CHN-II 2023

To better understand the TPH activity in the intestine, we decided to use a hybrid of in silico and in vivo experiments to investigate the TPH activity. The tryptophan hydroxylases (TPH) from human, as well as from mice, zebrafish, and rabbits have been selected and analyzed for their catalytic efficiency in the rate-limiting step of producing serotonin. By predicting the binding affinity of the TPHs to the tryptophan molecule we could predict the catalytic effectiveness of the enzyme selected.

In silico binding affinity prediction: The full-length 3D structures of the four TPHs from human, mouse, zebrafish, and rabbit are acquired as the PDB files from the uniport database under the following entry numbers: P17752; P09810; Q6PBX4; P17290 for each host organism respectively. For entry numbers P09810, Q6PBX4, and P17290, the known x-ray/NMR structures of the proteins are missing. Instead, the Alphafold predicted full-length structure of the proteins was selected for further analysis.

User Reviews

UNIQ1c58697d69c75a05-partinfo-00000005-QINU UNIQ1c58697d69c75a05-partinfo-00000006-QINU