Part:BBa_K4619010
PobR-Threshold Guard Switch
The gene pobR creates a transcriptional activator that attaches to the pobR operator on the dsDNA before combining with 4-HBA. Once 4-HBA is introduced to the solution, PobR binds with it and triggers the transcription of the dual pobA/R promoter on the side of pobA.
One of the advantages of this protein is its sensitivity and low leakage properties. Even tiny amounts of 4-HBA, at the micromolar level, can trigger transcription. This characteristic is crucial in creating a high-quality digitizer with a sharp response between two stable states(Calles et al., 2019).
Additionally, research indicates that most analogs of 4-HBA, such as p-aminobenzoate, can impede the activation of PobR, ensuring precise detection of 4-HBA.
Moreover, the combination of the non-activated PobR and pobR operator will inhibit the transcription of pobR when there is no 4-HBA stimulus, reducing the pressure on our bacteria.
Given PobR's excellent properties, we create a chimeric switch by combining it with a portion of the original threshold guard switch.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 462
Illegal AgeI site found at 154
Illegal AgeI site found at 675 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 595
Illegal SapI.rc site found at 1573
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