Part:BBa_K4779000

CMPG:A yeast copper-induced reporter pathway with prm1 promoter

This is a copper ion-responsive signaling pathway in Saccharomyces cerevisiae based on MAPK system. When the cells were induced by copper ions, the circuit with prm1 promoter activates GFP expression to indicate the copper ion concentration.

Construction

We obtained the gene sequences of the basic parts from IGEM, such as the promoter of Cup1 (BBa_K945002), MFα2 (BBa_K110005), the promoter of prm1 (BBa_K1346004), green fluorescent protein (GFP) (BBa_K3112009), and the terminator CYC1 (BBa_K4278703), and sent them to GENEWIZ (Suzhou Genewiz Biotechnology Co., Ltd.) for synthesis. The new composite part (CMPG) (BBa_K4779000) is also synthesized.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 279
  Illegal XbaI site found at 517
  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal PstI site found at 49
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal PstI site found at 49
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 55
  Illegal BamHI site found at 510
  Illegal XhoI site found at 8
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 279
  Illegal XbaI site found at 517
  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal PstI site found at 49
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 279
  Illegal XbaI site found at 517
  Illegal SpeI site found at 343
  Illegal SpeI site found at 523
  Illegal PstI site found at 49
  Illegal NgoMIV site found at 36
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2213
  Illegal SapI site found at 529