Part:BBa_K4613209

Locked Hairpin (LHP)

The locked hairpin is composed of the sequence of Ochratoxin A (OTA) aptamer, the sequence of RCA primer and the locked sequence. In the absence of OTA, the locked hairpin should be stable as "close status", eliminating nonspecific recognition. In the presence of OTA, the hairpin structure would transform into “open” conformation due to the recognition event between the aptamer and OTA. As shown in Fig. 2a (Lane 1 and Lane 2), RCA products was formed after adding the RCA primers. Then we explored the effect of different primers' concentrations on the amount of RCA products. Fig. 2b shows that within the same reaction time, the efficiency of RCA amplification increases with concentration.

Fig. 1 The principle of locked hairpin structure-switching.

Fig. 2 a. Verification of RCA Reation using Dumbbell Template. M: Marker. Lane2: negative control. Lane3: 100 μM RCA primer. b. Effect of different RCA primers' concentrations on the amount of RCA products. M: Marker. Lane2-7, different concentrations of RCA primers (0 to 100 μM).

Reference

1. Zhang J, Lu Y, Gao W, et al. Structure-switching locked hairpin triggered rolling circle amplification for ochratoxin A (OTA) detection by ICP-MS[J]. Microchemical Journal, 2023, 186: 108365.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]