Coding

Part:BBa_K4960040

Designed by: ShuChen Xi   Group: iGEM23_NUDT-CHINA   (2023-10-11)
Revision as of 15:39, 11 October 2023 by CirceTerry (Talk | contribs)


pPVC promoter->pvc1-pvc12->pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD->pvc14-pvc16

A system for targeted delivery of target proteins.

Usage and Biology

Mutant Pvc13-E01-DARPin fused with the DARPin E01 structural domain can enhance the binding ability of PVCs to cells, ultimately enabling targeted delivery of fat proteins. Pvc13-E01-DARPin mutant-based constructs of PVCs particles can specifically target cells with EGFR receptors[1]. In subsequent cell experiments, we used Hela and HEK-293T with high EGFR expression to ensure the targeting of pvc.

(A)
(B)
(A) Schematic diagram of the influence of different Linkers on the structure of tail fibers
(B) PVC illustration

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7406
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2407
    Illegal BglII site found at 11264
    Illegal BglII site found at 14099
    Illegal BglII site found at 15464
    Illegal BglII site found at 17422
    Illegal XhoI site found at 2691
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 11792
    Illegal NgoMIV site found at 17953
    Illegal NgoMIV site found at 18092
    Illegal AgeI site found at 6704
    Illegal AgeI site found at 9641
    Illegal AgeI site found at 10327
    Illegal AgeI site found at 10468
    Illegal AgeI site found at 11576
    Illegal AgeI site found at 18391
    Illegal AgeI site found at 20079
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None