Coding

Part:BBa_K4879003:Design

Designed by: Anuvind Pramod   Group: iGEM23_IISER-Pune-India   (2023-10-08)
Revision as of 12:24, 11 October 2023 by Sauron 3791 (Talk | contribs)


Yarrowia lipolytica HygR marker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.


Source

The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.

The DNA sequence, before optimization, was provided by our secondary PI Dr. Mridula Nambiar.

References